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. 2017 Aug 2;8:908. doi: 10.3389/fimmu.2017.00908

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Copyright © 2017 Forget, Tavera, Haymaker, Ramachandran, Malu, Zhang, Wardell, Fulbright, Toth, Gonzalez, Thorsen, Flores, Wahl, Peng, Amaria, Hwu and Bernatchez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimization of time and antibody concentration for T cell activation prior to transduction. (A) Peripheral blood mononuclear cell (PBMC) transduction efficiency following 24 and 48 h plate-bound anti-CD3 (OKT3) activation at different concentrations of antibody. (B) Tumor infiltrating lymphocyte (TIL) transduction efficiency following 24 and 48 h plate-bound anti-CD3 activation at different concentrations of antibody. (C) Fold expansion post-Rapid Expansion Protocol (post-REP) based on different concentrations of anti-CD3 antibody used for plate-bound activation (D) Transduction efficiency and CD3⁺CD8⁺ T cell percentages obtained following plate-bound anti-CD3 activation at different concentration of antibody at 48 h after transduction and day 14 of the REP.