Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 1;7:6990. doi: 10.1038/s41598-017-07421-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Analysis the auto secretion of sfGFP fusionsin E. coli. (a) Western Blot analysis of the extracellular expression of the sfGFP-PG4 in E. coli; C-T: total cell protein of the control; C-S: extracellular protein of the control; S1~S5: after expression, supernatant protein sample of culture media stayed in room temperature for one to five days; (b) SDS-PAGE analysis of the sub-fraction of the sfGFP-PG4 in E. coli; (c) SDS-PAGE analysis of the sub-fraction of sfGFP-Endo H in E. coli; (d) Enzyme activity analysis of sfGFP-Endo H; 1: native protein Rnase B; 2: native protein Rnase B digested by the recombinant protein Endo H (New England Biolab); 3~5: native protein Rnase B digested by sfGFP-Endo H (dilution 1:100, 1:1000, 1:10000); (e) SDS-PAGE analysis of the sub-fraction of sfGFP-ARG1 in E. coli; (f) Exclusion chromatography and SDS-PAGE analysis of sfGFP-ARG1; Lane 1: un-denatured sample of fusion protein sfGFP-ARG1; Lane 2: denatured sample of sfGFP; (g) SDS-PAGE analysis of the sub-fraction of sfGFP-GAD in E. coli; T: total cell proteins; S: supernatant of culture media; C: cytoplasmic protein; P: periplasmic protein; OM: outer membrane protein.