Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 1;7:7023. doi: 10.1038/s41598-017-07182-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Inhibition of inflammation in REDD1^−/− BMDM was mTORC1 independent. (a) REDD1^+/+ and REDD1^−/− BMDM were treated with rapamycin (Rapa) 40 nM for 45 minutes before being stimulated with LPS (100 ng/ml) for 20 minutes. Cell lysates were analyzed by immunoblots with indicated antibodies. (b) Quantification of phosphorylated proteins is shown (n = 3 independent experiments) with the value of REDD1^+/+ treated with LPS taken as 100 (c) REDD1^+/+ and REDD1^−/− BMDM were treated with rapamycin (Rapa) 40 nM for 45 minutes before being stimulated with LPS (100 ng/ml for 5 hours) and ATP (5 mM for 45 minutes). Cell lysates were analyzed by immunoblots with indicated antibodies. (d) Quantification of caspase-1 p20 normalized to tubulin is shown with the value of REDD1^+/+ treated with LPS taken as 100 (n = 3 independent experiments).