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. 2017 Aug 1;7:7023. doi: 10.1038/s41598-017-07182-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Oxidative stress was reduced in REDD1^−/− BMDM. REDD1^+/+ and REDD1^−/− BMDM were stimulated for 5 hours with LPS (100 ng/ml) followed by a treatment with ATP (5 mM) for 45 minutes. (a) ROS production was measured by oxidation of DCFH-DA with the value of REDD1^+/+ treated with LPS taken as 100. (b) mRNA expression was determined by quantitative RT-PCR (n = 3 independent experiments) * p < 0.05; **p < 0.01, ***p < 0.0001.