Table 2.

Twenty of the 28 genes detected in the farmed fish intestinal contents.

Classification of the antibiotics the genes confer resistance to	qPCR assay	Average relative abundance to the 16S rRNA gene
		Fish intestinal contents (sampled in 2014)	Fish farm sediments (sampled in 2006–2012)
Aminoglycoside	aadA1	2 × 10−2	a3 × 10−4
Aminoglycoside	aadA2-01	2 × 10−2	a2 × 10−4
Aminoglycoside	aadA2-02	2 × 10−2	a4 × 10−4
Aminoglycoside	aadA2-03	4 × 10−2	a8 × 10−4
Trimethoprim	dfrA1	6 × 10−2	a3 × 10−3
Trimethoprim	dfrA1-02	4 × 10−2	b1 × 10−3
Class 1 integron	intI1	6 × 10−2	b3 × 10−3
Other (Antiseptic)	qacEΔ1-01	1 × 10−2	a5 × 10−3
Other (Antiseptic)	qacEΔ1-02	1 × 10−1	a3 × 10−3
Sulfonamide	sul1	7 × 10−2	b4 × 10−3
Tetracycline	tet(32)	3 × 10−2	a1 × 10−3
Tetracycline	tetM-01	1 × 10−1	a3 × 10−3
Tetracycline	tetM-02	9 × 10−2	a2 × 10−3
Tetracycline	tetM-03	4 × 10−2	c1 × 10−3
Tetracycline	tetO-01	2 × 10−2	a2 × 10−3
Tetracycline	tetW-01	4 × 10−2	a4 × 10−4
Transposon	tnpA-01	3 × 10−2	a6 × 10−4
Transposon	tnpA-04	4 × 10−2	a2 × 10−4
Transposon	tnpA-06	5 × 10−1	a1 × 10−3
Transposon	tnpA-07	4 × 10−2	a4 × 10−3

^aMuziasari et al., 2016.

^bMuziasari et al., 2014.

^cTamminen et al., 2011.

^b,cThe quantification of the genes used standard qPCR.

The 20 genes were the same genes found to be enriched in the sediments below fish farms in the Northern Baltic Sea, Finland. The table also shows the average relative abundance of the genes to the 16S rRNA gene in the intestinal contents and in the farm sediments. The gene assays of the qPCR array grouped by classification of the antibiotics the genes confer resistance to, class 1 integron and transposon.