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Recombinant proteins of the two active HIs from cucumber can also use (Z,Z)-3,6-nonadienal as substrate. GLV-related GC-QTOF-MS extracted ion chromatogram (Ion 79) as output of the SPME-guided assay for measuring (3Z):(2E)-enal isomerase activity. Equal amounts of purified recombinant protein (2 μg each) of the two active HIs (Cs033090 and Cs078390) were tested. Recombinant protein of GST (EV) was used as control. (Z,Z)-3,6-nonadienal was used as substrate.
