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. 2017 Apr 20;24(9):2698–2706. doi: 10.1245/s10434-017-5858-7

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Programmed cell death protein 1 (PD1) expression on tumor-infiltrating lymphocytes (TILs) (a–c) and tumor cells (d–f) in esophageal adenocarcinoma detected by immunohistochemistry. a Negative PD1 staining of TILs. b 2+ (26–50%) positive staining of TILs. c 3+ (51–75%) positive staining of TILs. d Negative staining of tumor cells. e 2+ (26–50%) positive staining of tumor cells. f 4+ (75–100%) positive staining of tumor cells. The immunoreactivity for PD1 of tumor cells and TILs was examined at ×400 magnification, and the staining rate (percentage of tumor cells and lymphocytes showing positive staining, 0–100%) was determined. Arrows indicate examples for a positive PD1 staining on TILs (white arrow) and tumor cells (black arrow)