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. 2017 Aug 1;7:6983. doi: 10.1038/s41598-017-07557-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression in corneal endothelial cells and stromal cells after endothelial injury. ZO-1 gene expression in CECs after scraping injury was significantly higher than that in CECs after DM stripping at day 3, 7, and 14 (A). Na⁺/K⁺ ATPase gene expression significantly decreased after DM stripping at day 3, 7, and 14 (B). α-SMA gene expression in CECs dramatically increased after scraping injury and DM stripping at day 3, 7, and 14. α-SMA expression was higher in CEC scraping group at day 3, while was higher in DM stripping group at day 7 and day 14 (C). α-SMA gene expression in central corneal stroma showed mild increase in CEC scraping group at day 3, 7, and 14. While there was dramatic increase in DM stripping group at day 7 and day 14 (D). (*p < 0.05; **p < 0.01; ***p < 0.001).