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. 2017 Aug 1;7:6985. doi: 10.1038/s41598-017-07622-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ovarian cancer cell lines form tubular structures in 3D culture. Clear field images (magnification: 10×. Scale bar: 100 μm) and PAS stained images (magnification: 4×. Scale bar: 200 μm) of 3D cultures on matrigel at day 1 (left panels) and day 4 (right panels) in SKOV3, HEY, UCI101 and A2780 cells. At day 1 all cell lines form structures that could be described as capillary-like and are PAS+, however at day 4 only SKOV3 and HEY cell lines form cell aggregates surrounded by capillary-like structures. Tubular structures in SKOV3 and HEY were more PAS+ compared to the monolayer of cells, however in UCI101 and A2780, the individual cells stained strongly for PAS.