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. 2017 Aug 1;7:6985. doi: 10.1038/s41598-017-07622-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tubular Structures possess a glycoprotein-rich internal layer. (a) 3D reconstruction of the monolayer and tubular structures formed by the HEY-GFP ovarian cancer line upon PAS staining and analysis with laser confocal microscopy. PAS emits within the red spectrum upon laser excitation at 560 nm. (b) Reconstruction of the glyoprotein-rich component of a tubular structure. (c) Reconstruction of the glyoprotein-rich component surrounding a lumen. (d,e) Cross-section of PAS stained tubular structures. In red (panel d) a glycoprotein-rich component constructed from PAS staining. In green (panel e) cancer cells forming a tubular structure, (f) merge of the previous panels showing that the glycoprotein-rich component is present on luminal side of the green (cancer cell) tubular structures.