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. 2017 Aug 1;7:6985. doi: 10.1038/s41598-017-07622-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tubular structure formation in primary cultures derived from ovarian cancer patient ascites. (a) examples of tubular structure formation in primary cultures derived from ovarian cancer patients ascites at day 7 (a–c). For comparison, three cultures that did not demonstrate VM formation are shown (d–f) Magnification: 4×. Scale bar: 200 μm. (b) LYD microinjection into a tubular structure formed in 3D-culture primary culture on matrigel. The arrowhead indicates microinjection into an individual cell, demonstrating no dye movement. Magnification: 10×. Scale bar: 100 μm.