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. 2017 Aug 1;7:6991. doi: 10.1038/s41598-017-07233-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of adult neurogenesis between dominant and subordinate males in the hippocampus. Neurogenesis analysis was performed on dominant (Dom) and subordinate (Sub) male mice. (a) Experimental schedule. BrdU in drinking water (1.2 mg/mL) was supplied when the mice were aged 10–12 weeks, and the brains of the mice were collected when the mice were aged 12 weeks (n = 8 cages) for immunohistochemical analysis. Neurogenesis analysis was performed using antibodies against BrdU, and the neural cells were labelled with NeuN antibodies. DAPI was used to detect nuclei. Examples of the immunohistochemistry of samples from a (b) dominant and (c) subordinate mouse are shown. (d–f) Comparisons of (d) cell proliferation, (e) neurogenesis and (f) percentage of neurogenesis in newly formed cells in dominant and subordinate mice.