Fig. 1.

Analysis of Plexin-B2 expression in sensory neurons of the dorsal root ganglia (DRG) in adult mice and its regulation in inflammatory pain. a, b Expression of plxnb2 via immunofluorescence analysis of β-galactosidase in adult DRG in respective LacZ reporter knock-in mice. Typical examples a and quantitative analysis b of the distribution of DRG cell types expressing plxnb2 via co-immunolabeling with marker proteins (n = 10–20 sections/group taken from at least three different mice/group). Scale bar, 50 µm. c, d Typical examples c and quantitative summary d of LacZ staining demonstrating plxnb2 expression in adult Plexin-B2-LacZ^+/− mice following intraplantar injection of either vehicle (control) or Complete Freund´s Adjuvant (CFA) stimulating inflammatory pain (n = 20–30 sections/group taken from at least 3 different mice/group). Scale bars represent 50 µm. In d, Student’s t-test (two sides) was performed. P < 0.05 indicated by ^† as compared to basal. In all diagrams data are represented as mean, whereas error bars represent s.e.m. e, f Examples (left) and densitometric quantifications (right) of western blot analysis of PlexinB2 expression in paw tissue e or L3-L4 DRGs f in naive mice or at 24 h after intraplantar injection of CFA. Data are represented as fold-increase of the ratio of Plexin-B2 over housekeeping gene signal. All data are presented as mean ± s.e.m. n = 8 for paw tissues, n = 9 for DRGs. P < 0.05 indicated by ^† as compared to basal