Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 1;7:6970. doi: 10.1038/s41598-017-06718-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The expression of PRDX2 inhibit hypoxia-mediated oxidative stress injury to H9c2. (A) H9c2 cells were transfected with PRDX2 siRNA (siPRDX2) and control siRNA (siSCR) at a concentration of 80 pmols. Cells were harvested 36 h after the transfection, and the silencing effect on PRDX2 mRNA levels was determined by RT-PCR. The silencing effect of PRDX2 siRNA at the protein level was determined 48 h post-transfection using western blotting. The data are presented as means ± SD of the mean from five separate cell experiments. ***p < 0.001 vs control. (B) H9c2 cells were transfected with a PRDX2 overexpression vector and control (SCR). Cells were harvested 48 h after the transfection, and the PRDX2 mRNA levels were determined by RT-PCR. The overexpression effect of PRDX2 at the protein level was determined 48-h post-transfection using western blotting. GAPDH was used as a loading control. The data are presented as means ± SD of the mean from five separate cell experiments. ***p < 0.001 vs control. (C and D) Mitochondrial ROS production in H9c2 cells after culture in normal hypoxic conditions for 6 h with/without PRDX2 overexpression or down-regulation. The data are presented as means ± SD of the mean from ten independent views. ***p < 0.001 vs control. Magnification 200×. (E) The effects of PRDX2 on hypoxia-induced H9c2 cell viability were detected after exposed to hypoxia medium for 6 h. The data are presented as means ± SD of the mean from three separate cell experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs control. (F) Western blot analysis of Bcl-2, Bax, Caspase-3. GAPDH was used as a loading control. (G–I) Statistical analysis of band intensity for Bcl-2, Bax, and Caspase-3. The data are presented as means ± SD of the mean from three separate cell experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs control.