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. 2017 Aug 2;4:17039. doi: 10.1038/hortres.2017.39

Figure 5.

Figure 5

Analysis of transcriptional activity of HpWRKY44. (a) Reporter and effector constructs. The dual luciferase reporter construct contained the firefly luciferase (LUC) reporter gene fused with five copies of the GAL4 DNA-binding element (5×GAL4) plus the mini-35S (TATA box). The Renilla luciferase (REN) driven by CaMV 35S in the same vector was used as an internal control. The effector plasmid contained the HpWRKY44 gene fused to the yeast GAL4 DNA-binding domain (GAL4BD) driven by CaMV35S, CPMV, (Cowpea mosaic virus). (b) Transcriptional activation activity of HpWRKY44. The trans-activation ability of HpWRKY44 was assessed as the ratio of LUC to REN. Each presented value represents the mean±s.e.m. six biological replicates (n=6). The ratio of LUC/REN of the empty pBD vector was used as calibrator and set at 1. ** indicates a significant difference between the sample (transcriptional activator vector) and the control (empty pBD vector) at P<0.01, based on the Student’s t-test.