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The growth defect of the nos mutant does not result from depletion of intracellular α-ketoglutarate. (A and B) Growth rates of S. aureus wild-type and mutant strains were monitored for 12 h in the absence (A) or presence (B) of 25 mM α-ketoglutarate (means ± SEM, n = 4). (C) α-Ketoglutarate levels were determined from culture supernatants of wild-type (WT) and mutant strains by HPLC (representative trace, n = 2). AU, arbitrary units.
