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. 2017 Aug 1;8(4):e01060-17. doi: 10.1128/mBio.01060-17

TABLE 1 .

Tn-seq analysis statisticsa

Biological replicate
and sample
Total no. of reads % reads with
no Tn
sequence
No. of reads
too short
No. of reads
used for
analysis
ARb (%) NFc Total no. of
normalized
reads
No. of
reads/Tn site
Biological replicate 1
    Input 21,652,251 5.20 36,631 20,489,022 80.40 0.844 18,274,500 129
    Nontreated 20,304,098 6.94 29,769 18,865,247 80.09 0.920 18,679,770 119
    NCR247 treated 20,121,669 8.14 34,245 18,450,409 80.80 0.921 18,532,057 116
    PMB treated 18,401,067 7.18 30,931 17,049,767 80.66 0.998 18,364,265 107
Biological replicate 2
    Input 20,153,000 12.72 30,042 17,560,139 81.50 0.943 19,004,279 111
    Nontreated 22,731,094 7.97 33,510 20,885,738 80.66 0.811 18,434,917 132
    NCR247 treated 18,798,923 7.77 30,051 17,307,732 80.11 1.000 18,798,923 109
    PMB treated 18,906,038 8.75 28,138 17,224,367 79.88 0.994 18,792,602 109
a

Tn, transposon.

b

AR, alignment rate.

c

NF, normalization factor as determined by normalizing all samples to the sample with the lowest number of intergenic reads.