FIG 1 .

Fewer Tregs in B cell-deficient mice. Spleens were harvested from naive C57BL/10 mice (A to E) or B cell-deficient μMT mice (B to E), and CD4, CD8, CD19, Ki-67, and FOXP3 expression was analyzed by flow cytometry. (A) Representative gating strategy for CD4⁺ T cells (top) or CD8⁺ T cells (bottom). (B) Quantification of the percentage of FOXP3⁺ cells within the CD4⁺ T cell gate. (C) Quantification of the percentage of Ki-67⁺ cells within the Treg (CD4⁺ FOXP3⁺) gate. (D) Quantification of the percentage of CD8⁺ cells within the non-B cell lymphocyte gate (CD19⁻ lymphocytes). (E) Quantification of the percentage of Ki-67⁺ cells within the CD8⁺ T cell gate. Each dot in panels B to E represents an individual mouse pooled from experiments performed with 4 to 5 mice per group. Lines indicate the means of data for the group. In panels B to E, statistical significance was determined with a Student’s t test; ns, = P > 0.05.