FIG 4 .
Enhanced anti-FV CD8+ T cell responses in B cell-deficient mice. (A to F) C57BL/10 (B10) mice and μMT mice were infected with 20,000 SFFU of FV i.v. or left naïve, spleens were harvested at 2 weeks (A to F) or 3 weeks (B to F) postinfection, and CD8, GrB, CD11a, and Db-GagL dextramer binding was analyzed by flow cytometry. (A) Representative gating strategy for CD8+ T cells at 2 wpi. (B to F) Quantification of the percentage of dextramer+ (B), percentage of dextramer+ GrB+ (C), absolute number of dextramer+ GrB+ (D), percentage of CD11a+ GrB+ (E), and absolute number of CD11a+ GrB+ (F) cells among splenic CD8+ T cells. In panels B to F, each dot represents an average of 5 to 12 mice from pooled experiments and error bars show ± SEM. *, P < 0.05; **, P < 0.01 (comparing B10 mice to μMT mice at the indicated time point, as determined by one-way ANOVA with Tukey's posttest for multiple comparisons).