. 2017 Jun 25;17(7):1495. doi: 10.3390/s17071495

Table 1.

Summary of DMF platforms for nucleic acid amplification. ITO—Indium–Tin–Oxide; PCB—Printed Circuit Board; PEN—Polyethylene Naphthalate; qRT-PCR—Quantitative Real-Time Polymerase Chain Reaction; SNP—Single Nucleotide Polymorphism; POC—Point-of-care; RCA—Rolling Circle Amplification; C2CA—Circle-to-circle Amplification; RPA—Recombinase Polymerase Amplification; MRSA—methicillin-resistant Staphylococcus aureus (MRSA); M pneumoniae—Mycoplasma pneumoniae; C. albicans—Candida albicans; E. coli—Escherichia coli; P. aeruginosa—Pseudomonas aeruginosa.

	Application	Reaction Volume	Actuation Voltage	Dielectric Material	Hydrophobic Material	Electrode Material	Substrate Material	Filler	Reference
DMF–PCR platforms	Dengue II virus detection	1.46 µL	12 V_RMS (3 kHz)	Si₃N₄	Teflon^® AF	Au	Glass	Silicone oil	[17]
	SNP genotyping	64 nL	60 V_RMS (3 kHz)	Si₃N₄	Teflon^® AF	Au	Glass	Silicone oil	[25]
	POC testing, MRSA, S. aureus and C. albicans detection	600 nL	-	Parylene C	Teflon^® AF	Cr	PCB	Hexadecane	[9]
	MRSA, S. aureus, M. pneumoniae and C. albicans detection	Variable	-	-	-	-	PCB	Hexadecane/silicone oil	[21]
	S. aureus detection	1.2 nL	70 V–250 V	Parylene C	Teflon^® AF	-	-	n-dodecane	[23]
	Cell genetic expression analysis	Variable	48 V (3 kHz)	Si₃N₄	SiOC	Ti/AlCu	Silicon wafer	Silicone oil	[24]
	Bacteriophage M13mp18 detection	1.5 µL	80–100 V_RMS (18 kHz)	Solder mask	Teflon^® AF	Cu/Ni/Au	PCB	Air	[22]
Validation of DMF–PCR platforms	C. albicans detection on human blood	Variable	-	-	-	-	PCB	Hexadecane/silicone oil	[27]
Validation of DMF–PCR platforms	M. pneumoniae detection on human nasopharyngeal wash	Variable	-	-	-	-	PCB	Hexadecane/silicone oil	[28]
Hybrid platforms	DNA amplification (only the primers are described)	25 µL	-	SiO₂	Teflon^® AF	Cr/Au	Glass	Silicone oil	[31]
Hybrid platforms	Pre-DNA sequencing	2.8 µL	80–90 V_RMS (15 kHz)	Parylene C	Teflon^® AF	ITO	Glass	Air	[32]
DMF platforms for isothermal amplification	CTX-M gene detection in E. coli bacteria	Minimum 270 nL	20 V	Al₂O₃	Cytop^®	ITO	Glass	n-dodecane	[18]
DMF platforms for isothermal amplification	P. aeruginosa detection	5 µL	120 V_AC (1 kHz)	Parylene C	Teflon^® AF	Cr	Glass	Vapor-Lock oil	[19]