Fig. 2.

Fmo5^−/− mice are resistant to weight gain and changes in glucose homeostasis in response to a high-fat diet. (A) Body weight of WT and Fmo5^−/− (KO) mice fed a high-fat diet (WT, n = 7; KO, n = 6). (B) Plasma insulin concentrations of WT and KO mice fed standard chow (SC) (n = 3) or fed a high-fat diet (HFD) for 6 weeks (WT, n = 3; KO, n = 4). (C) GTT of WT and KO mice fed an SC diet (n = 5) or an HFD for 6 weeks (n = 4). Insert shows the AUC. (D) Western blot analysis of fecal proteins from WT mice fed an SC diet or an HFD for 6 weeks. Each lane represents proteins from a different mouse. M, molecular mass standards. The blot was incubated with an antibody against FMO5 and was developed as described in Materials and Methods. Protein loading was assessed as described in Materials and Methods. (E) Western blot analysis of liver proteins from WT mice fed an SC diet or an HFD for 6 weeks. Each lane represents proteins from a different mouse. The blot was incubated with an antibody against FMO5 or actin as the internal loading control. The blot was developed as described in Materials and Methods. Data in (A), (B), and (C) are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.