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. 2017 Jul 1;17(7):1545. doi: 10.3390/s17071545

Figure 2.

Figure 2

Characterization of CF680R-actin in live XTC cells. (A) Photostability of CF680R-actin using excitation light with the 75 W xenon illumination system in live cells. The excitation light used was full (100%) or attenuated to 50%, 25%, 12% or 6% as indicated. Black lines show the single-exponential curve fitted to the data. The data shown are representative of three independent experiments; (B) Dispersion of the central position of the immobile glass-bound CF680R-actin speckle acquired with a 200 ms exposure time and a full 75 W xenon excitation (fast-tracking images, 100 frames). The standard deviations of the centroid positions in one direction are 16.3 nm for the x-axis and 16.0 nm for the y-axis. The data shown are representative of the data from three independent experiments; (C) Displacement plot of the central position of CF680R-actin in lamellipodia in the series of fast-tracking images (50 frames). Note that CF680R-actin speckles moved with the retrograde actin flow in lamellipodia. The velocity of the CF680R-actin speckles was calculated from a linear fit. The data shown are representative of the data from three independent experiments.