FIGURE 3.

Identification of overexpressed strains. Total RNA was isolated from leaves of Arabidopsis seedlings in culture. Basta screening in overexpressing strains of Arabidopsis (A); Genomic DNA PCR of overexpressed strains, lane 1 blank control, with ddH₂O as the template; lane 2 negative control WT, with WT DNA as the template; lanes 3–9 different overexpressed strains (B); The transgenic plants were tested by real time PCR, the Arabidopsis actin gene was used as a reference gene. S3-S35 indicates the different transgenic Arabidopsis strains while WT indicates the WT Arabidopsis (C); Relative expression levels of SsGPAT in S14 and S17 when treated with 0, 50, and 100 mM NaCl (D). Each column represents the means ± SD of three measurements (n = 3). Means identified by different letters are significantly different at P ≤ 0.05.