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. 2017 Aug 2;8:1337. doi: 10.3389/fpls.2017.01337

FIGURE 4.

FIGURE 4

Identification of T-DNA insertion Arabidopsis mutant. The T-DNA is forward inserted at the intron of At2g38110 (gpat6) and reversely inserted at the intron of At1g02390 (gpat2) genomic locus (A). T-DNA insertion was confirmed using PCR with the indicated primer sets, and the M refers to the 2000 bp marker. Lanes 1–8 gpat2 and lane 9 WT (B). Lanes 1–10 gpat6 and lane 12 WT (C). AtGPAT transcripts were determined by qPCR (D). Data are presented as the means ± SD of three replicates (n = 3). Different letters indicate a significant difference at P ≤ 0.05.