Table 4.
The effect of log transformation using non-normalized data.
| No Log Transform Normalized, Missing replaced with zero (63% of identified spots picked up) | Log Transformed Normalized, Missing replaced with – 17.28 (100% of identified spots picked up) |
| SSP 1134 | |
| SSP 1509 | SSP 1509 |
| SSP 1733 | |
| SSP 2309 | SSP 2309 |
| SSP 3219 | |
| SSP 3806 | |
| SSP 4203 | |
| SSP 4225 | SSP 4225 |
| SSP 4435 | SSP 4435 |
| SSP 4519 | |
| SSP 4724 | |
| SSP 5309 | |
| SSP 5329 | |
| SSP 5413 | SSP 5413 |
| SSP 6205 | SSP 6205 |
| SSP 6228 | |
| SSP 6304 | SSP 6304 |
| SSP 6314 | SSP 6314 |
| SSP 6321 | |
| SSP 6349 | |
| SSP 6443 | |
| SSP 6452 | SSP 6452 |
| SSP 7223 | SSP 7223 |
| SSP 7334 | |
| SSP 7750 | SSP 7750 |
| SSP 8613 |
Column 1 has spots that have significantly different intensities (p = 0.05) after normalizing and log transforming data. Column 2 has spots that are significantly different in intensity after normalizing data (using normalization 2), but after log transformation. The percentages in parenthesis in the header measure how many of the ten proteins known to be different were identified after log transformation and normalization.