Table 2.
Comparison of targeting signals and subcellular localizations of AcCATPO predicted and N- and C-terminal GFP tagged.
| Gene name | Length (AA) | Cell localization (this study) |
Cell localization- bioinformatic prediction |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| C-TERM | N-TERM | WoLF PSORT II_Over 2 | ProtComp 9.0 _ Over 1 |
CELLO V2.5 |
|||||||||
| LDB | PLDB | NN | PT | ALL | LOC | RC | |||||||
| AcCATPO | 492 | NUC/CYT/PER | NUC/PER | PER | 7 | PER | 3.1 | 1.2 | 0.96 | 8.12 | 3.53 | PER | 4.0 |
| MIT | 4 | EC | 1.9 | 0.7 | 0.96 | 0.05 | 2.28 | MIT | 0.4 | ||||
| CHL | 2 | CYT | 2.0 | 1.2 | 0 | 0 | 2.02 | CYT | 0.2 | ||||
| MIT | 3.0 | 0 | 0 | 0 | 2.00 | ||||||||
AA, amino acids; C-TERM, C-terminal-tagged GFP; N-TERM, N-terminal-tagged GFP; NUC, nucleus; CYT, cytoplasm; PER, peroxisome; MIT, mitochondria; CHL, chloroplast; EC, extra cellular; LDB, localization from database; PLDB, predicted location from database; NN, neural networks; PT, pentamers; ALL, combined results from LDB, PLDB, NN, and PT; LOC, prediction of location; RC, reliability class.