Figure 2.

Binding of immunoglobulin G (IgG) glycoforms to human FcγR. Binding of IgG glycoforms to the human FcγR family as determined by surface plasmon resonance, displayed as relative binding compared to unmodified IgG1 (U), (A) FcγRI, (B) FcγRIIa 131H, (C) FcγRIIa 131R, (D) FcγRIIb/c, (E) FcγRIIIa V158, (F) FcγRIIIa F158, (G) FcγRIIIb NA1, and (H) FcγRIIIb NA2. x-Axis legend describes the percentage of each derived glycan trait indicated and by grayscale, from light to dark. The data represent the mean and SEM of at least two combined independent experiments; *, **, ***, and **** (above each column as tested against unmodified, or as indicated, for FcγRIIIs comparing each set of five glycoforms defined by the vertical dotted lines, based on fucose and bisection levels) denote a statistical significance of p ≤ 0.05, p ≤ 0.01, p ≤ 0.001, and p ≤ 0.0001, respectively, as tested by one-way ANOVA using Tukey’s multiple comparisons test. U: unmodified glycoform.