Figure 4.

Complement activation by glyco-engineered anti-TNP IgG1. Relative (A) binding of C1q (n = 4) and (B) C4 deposition as determined by ELISA (n = 4), (C) complement-mediated lysis of aTNP opsonized red blood cells (n = 3). Data represent the mean and SEM of combined independent experiments; *denotes a statistical significance of p ≤ 0.05, as tested by a one-sample t-test against a theoretical mean of 100 (%). x-Axis legend describes the percentage of each derived glycan trait indicated and by grayscale, from light to dark. U: unmodified glycoform.