Table 1.

Comprehensive list of glycopeptide degrees of complex glycans found in the glyco-engineered IgG1 batches of anti-D and anti-TNP specificity.

2FF, 0.4 mM 2-deoxy-2-fluoro-l-fucose; 2FG, 1 mM 2-deoxy-2-fluoro-d-galactose; GNT3, co-transfection of 1% GNT3 vector; B4GALT1/d-galactose, co-transfection of 1% B4GALT1 vector and addition of 5 mM d-galactose; ST6GALT, co-transfection of 2.5% ST6GALT vector; in vitro sialylation, treatment of sample with recombinant ST6GALT and CMP-NANA; G, number of galactoses; F, presence of a core fucose; N, presence of a bisecting N-acetylglucosamine; S, number of N-acetylneuraminic (sialic) acids.

These resulted in significantly different derived glycosylation traits (fucosylation, bisection, galactosylation, sialylation, high-mannose, hybrid-type), which are calculated from the relative abundances of individual N-glycans.

The shading of cells indicates, for each glycoform the lowest to highest abundance of glycopeptides, respectively, from light to dark.

^aFor complex immunoglobulin G glycans, we use a nomenclature which assumes structural knowledge of the glycans based on literature.