Table 1.
Primary antibodies used for immunohistochemical staining
| Antibody target | Source | Code number | Working dilution | Type of antibody |
|---|---|---|---|---|
| Msi-1 | Novus Biologicals Europe (Abingdon, UK) | NB100–1759 | 1:100 | Polyclonal, raised in rabbit against residues 5–21 [APQPGLASPDSPHDPCK] of human, mouse, and rat Msi-1 |
| NEUROG3 | ThermoFisher Scientific (Oslo, Norway) | BT-B56180 | 1:50 | Polyclonal, raised in rabbit against KLH-conjugated synthetic peptide at 40–69 amino acids from the N-terminal region of human NEUROG3 |
| CgA | Dako (Glostrup, Denmark) | M869 | 1:1000 | Monoclonal, raised in mouse against the N-terminal of purified CgA |
| Serotonin | Dako | 5HT-209 | 1:1500 | Monoclonal, raised in mouse against serotonin |
| PYY | Alpha-Diagnostica (San Antonio, TX, USA) | PYY 11A | 1:1000 | Polyclonal, raised in rabbit against PYY |
| Oxyntomodulin (enteroglucagon) | Acris Antibodies (Herford, Germany) | BP508 | 1:800 | Polyclonal, raised in rabbit against porcine glicentin/glucagon |
| PP | Diagnostic Biosystems (Pleasanton, CA, USA) | #114 | 1:400 | Polyclonal, raised in rabbit against synthetic human PP |
| Somatostatin | Dako | A566 | 1:200 | Polyclonal, raised in rabbit against synthetic human somatostatin |