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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Exp Neurol. 2017 Apr 12;293:144–158. doi: 10.1016/j.expneurol.2017.04.004

Figure 3.

Figure 3

Carrageenan-induced AMPAr trafficking and membrane insertion of Ca2+ permeable AMPAr is PI3K dependent. (A) Pretreatment with i.t. LY294002 (LY) blocks carrageenan-induced trafficking of GluA1 to the plasmalemma in ipsilateral dorsal horn. (B) Injection of LY prior to paw injection leads to a pronounced reduction in plasmalemma GluA2, which is not observed in non-inflamed animals. (C–E) Representative examples of Co2+ uptake induced by kainate in lumbar spinal cord sections incubated with vehicle, TNF, or Wortmannin (Wort), a non-selective PI3K antagonist. (F) Quantification of cells with positive Co2+ staining shows increased Co2+ uptake in both superficial and deep lumbar dorsal horn sections after TNF incubation, which is prevented Wort. Data is presented as mean±SEM, n=3, * p < 0.05, ** p < 0.01