Table 2.

Regional brain volumes variance explained by treatment (Vehicle vs Sucrose) in CPCA modeling.

Brain Structure	PC1		PC2
	Loading	P-value	Loading	P-value
Cerebrum / Cerebral cortex	0.279	0.0021	0.820	0.0000
Amygdalo piriform transition area	0.255	0.0004	0.685	0.0000
Caudo medial entorhinal cortex	0.297	0.0070	0.665	0.0000
Postero lateral cortical amygdaloid area	0.312	0.0013	0.554	0.0000
Postero medial cortical amygdaloid area	0.225	0.0017	0.748	0.0000
Rostral amygdalo piriform area	0.271	0.0092	0.525	0.0000
Ventral tenia tecta	0.191	0.0292	0.399	0.0000
Cerebrum / Cerebral nuclei
Bed nucleus of stria terminalis	0.279	0.0021	0.820	0.0000
Brainstem
Medulla	0.203	0.0094	0.351	0.0002
Superior olivary complex	0.061	0.4517	0.370	0.0001
Cerebellum
Lobules 4–5: culmen (ventral and dorsal)	0.329	0.0004	0.536	0.0000
Lobule 7: tuber (or folium)	0.185	0.0129	0.418	0.0000
Lobule 8: pyramis	0.190	0.0233	0.439	0.0000
Copula pyramis lobule 8	0.271	0.0020	0.427	0.0000
Trunk of lobules 6–8 white matter	0.265	0.0009	0.391	0.0000
Lobule 8 white matter	0.110	0.1854	0.394	0.0001
Lobule 9: uvula	0.266	0.0006	0.507	0.0000
Lobule 9 white matter	0.201	0.0106	0.484	0.0000
Lobule 10: nodulus	0.260	0.0015	0.433	0.0000
Flocculus FL	0.156	0.0549	0.509	0.0000
Flocculus white matter	0.099	0.2381	0.397	0.0000
Paraflocculus PFL	0.250	0.0019	0.329	0.0002
Ventricular system
Third ventricle	0.410	0.0010	0.483	0.0001

CPCA factor loadings for analysis of treatment effect on regional brain volumes. Component loadings for PC1 and PC2 and their P-values for the 22 brain regions that passed the Bonferroni cut-off (P < 0.013) are listed. Treatment (represented in PC2) loaded uniquely on 22 brain regions, reflecting that mice exposed to Sucrose have significantly smaller volumes in these specific brain areas compared to Vehicle (P < 0.013), n per group 52–57.

CPCA, constrained principal component analysis; PC, principal component.