Table 4.

Effects of exposure to interventions on adult mice regional brain volumes within the 3 groups: CPCA analysis split by treatment - Vehicle.

Brain Structure	PC1		PC2		PC3
Brain Structure	Loading	P-value	Loading	P-value	Loading	P-value

*Cerebrum*
*Cerebral cortex/cortical plate*
Cingulate cortex area 30	−0.572	0.0000	0.398	0.0000	−0.359	0.0004
Secondary auditory cortex dorsal area	−0.594	0.0000	0.386	0.0002	−0.331	0.0005
Caudomedial entorhinal cortex	−0.096	0.3225	0.510	0.0001	−0.376	0.0006
Cingulum	−0.566	0.0000	0.358	0.0001	−0.291	0.0010
Ectorhinal cortex	−0.608	0.0000	0.446	0.0000	−0.311	0.0147
Postero lateral cortical amygdaloid area	−0.330	0.0050	0.488	0.0000	−0.318	0.0216
Perirhinal cortex	−0.525	0.0000	0.410	0.0000	−0.319	0.0054
Rostral amygdalo piriform area	−0.169	0.1585	0.548	0.0000	−0.300	0.0072
Temporal association area	−0.540	0.0000	0.400	0.0002	−0.271	0.0361
Primary visual cortex	−0.519	0.0000	0.490	0.0000	−0.333	0.0010
Secondary visual cortex lateral area	−0.625	0.0000	0.425	0.0000	−0.332	0.0006
Hippocampus	−0.433	0.0001	0.670	0.0000	−0.351	0.0004
Dentate gyrus of hippocampus	−0.241	0.0875	0.602	0.0000	−0.264	0.0259
Stratum granulosum of hippocampus	−0.318	0.0191	0.587	0.0000	−0.241	0.0507
*Cerebral nuclei*
Lateral septum	−0.671	0.0000	0.372	0.0000	−0.287	0.0065
Medial septum	−0.547	0.0000	0.487	0.0001	−0.230	0.0481
Nucleus accumbens	−0.669	0.0000	0.405	0.0000	−0.252	0.0055
*Brainstem*
Midbrain	−0.538	0.0000	0.518	0.0000	−0.243	0.0286
*Cerebellum*
*Cerebellar cortex/vermis*
Lobule 3 central lobule dorsal	−0.260	0.0048	0.540	0.0001	−0.215	0.0987
Lobule 3 white matter	−0.285	0.0027	0.483	0.0001	−0.292	0.0308
Lobules 4–5 culmen ventral and dorsal	−0.161	0.2575	0.493	0.0002	−0.163	0.2286
Lobule 7 tuberor folium	−0.083	0.4901	0.528	0.0000	−0.043	0.7456
Lobule 8 pyramis	−0.221	0.0430	0.517	0.0002	−0.006	0.9641
Lobule 9 uvula	−0.285	0.0065	0.566	0.0001	−0.122	0.3305
*Cerebellar cortex/hemispheres*
Simple lobule 6	−0.533	0.0000	0.484	0.0000	−0.121	0.3051
Crus 1 white matter	−0.478	0.0000	0.525	0.0000	−0.304	0.0005
Crus 2 ansiform lobule 7	−0.479	0.0000	0.530	0.0000	−0.185	0.0301
Crus 2 white matter	−0.541	0.0000	0.448	0.0000	−0.267	0.0033
Paramedian lobule 7	−0.471	0.0000	0.503	0.0002	−0.056	0.6141
Trunk of crus 2 and paramedian white matter	−0.532	0.0000	0.404	0.0002	−0.192	0.0482
Flocculus FL	−0.120	0.2667	0.592	0.0000	−0.077	0.5402
*Fiber Tracts*
*Cranial nerves*
Lateral olfactory tract	−0.440	0.0000	0.385	0.0002	−0.413	0.0047
Medial lemniscus medial longitudinal fasciculus	−0.338	0.0006	0.536	0.0002	−0.172	0.1684
*Lateral forebrain bundle system*
Corticospinal tract pyramids	−0.197	0.0567	0.493	0.0002	0.038	0.7919
*Extrapyramidal fiber system*
Ventral tegmental decussation	−0.506	0.0000	0.609	0.0000	−0.184	0.0607
*Medial forebrain bundle system*
Fimbria	−0.552	0.0000	0.360	0.0001	−0.160	0.1743
Fornix	−0.565	0.0000	0.454	0.0000	−0.272	0.0133
Fasciculus retroflexus	−0.645	0.0000	0.325	0.0000	−0.152	0.1625
Stria medullaris	−0.665	0.0000	0.338	0.0002	−0.087	0.4835
*Other*
Pre-para subiculum	−0.249	0.0171	0.495	0.0002	−0.371	0.0077

Component loadings for PC1, PC2 and PC3 and their P-values for the 40 brain regions that passed the Bonferroni cut-off (P < 0.013; shown in bold font) for significance are listed; n = 16–18 per group. Brain regions are presented by anatomical distribution according to Allen Mouse Brain Atlas.

Significant P-values CPCA, constrained principal component analysis; PC, principal component.