Figure 5.

Route of micelleplex cellular uptake was investigated in human lung adenocarcinoma epithelial cells (A549) following a 1 h treatment with the various endocytosis inhibitors nystatin (10 μg ml⁻¹), wortmannin (12 ng ml⁻¹), and methyl-β-cyclodextrin (3 mg ml⁻¹). Median fluorescence intensity was measured via flow cytometry after 4 h transfection with micelleplexes assembled via batch reactor (A) or microfluidic mixing (B) at an N/P ratio of 6 with 50 pmol AF488 siRNA. Data points indicate mean ± SD (n = 3). One-way ANOVA ^***P < 0.001.