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In vivo GAPDH gene knockdown efficiency was validated in whole lung RNA isolated from normal BALB/c mice that were intratracheally instilled with micelleplexes assembled via batch reactor and microfluidic mixing at an N/P ratio of 6 with 750 pmol msGAPDH siRNA. GAPDH expression was normalized with β-actin expression and quantified by real time PCR. Data points indicate mean ± SD (n = 4). One-way ANOVA, *P < 0.01, ***P < 0.001.
