Table 4.

Effects of PNA administration on X-linked gene expression as measured by RNA-FISH experiments

	Mecp2 single dot	Mecp2 double dot	Number nuclei	Percent single dot	Percent double dot	(+) Xist bodies	(−) Xist bodies	Number nuclei	Percent Xist bodies
(−)PNA	288	264	552	52	48	332	220	552	60
(+)PNA	65	415	480	13	87	50	430	480	10

To quantitate the response of cells to PNA–transportan (pWS1248) treatment, we counted the cells with Xist bodies, one or two sites of expression for either Pgk1 or Mecp2, as measured by RNA–FISH. The total number of cells counted, number of cells with and without Xist bodies, and the number of cells with one or two spots of expression for Mecp2 are presented.