Table 5.

Effects of PNA administration on X-linked gene expression as measured by RNA-FISH experiments

	Pgk1 single dot	Pgk1 double dot	Number nuclei	Percent single dot	Percent double dot	(+) Xist bodies	(−) Xist bodies	Number nuclei	Percent Xist bodies
(−)PNA	241	277	518	46	53	295	223	518	57
(+)PNA	122	396	518	23	76	64	454	518	12

To quantitate the response of cells to PNA–transportan (pWS1248) treatment, we counted the cells with Xist bodies, one or two sites of expression for either Pgk1 or Mecp2, as measured by RNA–FISH. The total number of cells counted, number of cells with and without Xist bodies, and the number of cells with one or two spots of expression for Pgk1 are presented.