Figure 2.

Analysis of brain-infiltrating lymphocytes of mice. Mice were intraperitoneally injected with anti-Tim-3 and/or anti-CEACAM1 mAbs or control mAbs on days 12, 15, and 18. On day 24, the BILs of mice in the different groups were analyzed by flow cytometry. (A) The proportion of CD4⁺ T cells in the brains of mice. (B) The percentage of CD8⁺ T cells in the brains of mice. (C) The proportion of CD4⁺CD25⁺FoxP3⁺ Treg cells in the brains of mice. (D, E) Ratios of CD4⁺ and CD8⁺ T cells to Tregs. (F, G) The absolute numbers of CD4⁺, CD8⁺ T cells in the brains of mice. All experiments performed in duplicate. *** P<0.001; ** P<0.01, compared with control mice.