CIH induces apoptosis in human cardiomyocyte cell line H9C2. (A). Representative flow cytometry scatter plots of propidium iodide (PI) (Y axis) vs. annexin V-fluorescein isothiocyanate (FITC) (X axis) and (B). Quantitation data of the average of 3 independent flow cytometry experiments. (C). Effect of CIH on apoptosis protein expression of BimEL, BimL, BimS, BAX, Bcl2, and caspase3 by Western blot analysis. (D) and (E) Quantitation data of (C). * P<0.05, ** P<0.01 compared with control, n=3.