Figure 4.

FOXO1 mediates Bim expression in response to CIH. (A) H9C2 cells were transfected with lentiviral vector and subjected to Western blot analysis to identify FOXO1 or Bim expression. (B) Quantitation data of (A). (C) H9C2 cells were transiently transfected with FOXO1 siRNA or a control RNAi and then treated with CIH. The cells were then harvested and subjected to Western blot analysis to identify the expression of FOXO1 and Bim. (D) Quantitation data of (C). (E) The potential interaction between FOXO1 and Bim was investigated via co-immunoprecipitation and immunoblotting in H9C2 cells exposed to either normoxia or CIH. (F) Full-length blots of FOXO1 and Bim and GAPDH are presented. (G) The expression levels of FOXO1 and Bim were quantified by immunoblots in HPASMCs transfected with siRNA exposed to CIH. (H) Results were quantified by densitometry analysis of the band form (G) and then normalization to GAPDH protein. * P<0.05, ** P<0.01 compared with control, n=3.