Table 1.

Sequencing statistics for calculating enrichment ratios of the different panning samples. For each data set the total number of sequencing reads, the number of reads that passed quality control and further processing, as well as the number of observed versus the expected number of single NNK mutations in all three CDRs of given sample are listed.

Library name	Panning Antigen	Panning type	Final panning condition	Library	No. of raw reads	Reads after filtering and quality control
T28P_HCNNK_presort	—	—	—	HC	3105123	890518
T28P_HCNNK_V0.2	VEGF	Solution	0.2nM VEGF @ RT	HC	2909108	1848223
T28P_HCNNK_V0.2comp37	VEGF	Solution	0.2nM VEGF +1000x competition @ 37°C	HC	2821201	1835315
T28P_HCNNK_A20.2	Ang2	Solution	0.2nM Ang2 @ RT	HC	1250730	794404
T28P_HCNNK_A20.2comp	Ang2	Solution	0.2nM Ang2 + 1000x competition @ RT	HC	1980579	1213739
T28P_HCNNK_A1	Ang1	Plate	5ug/ml Ang1 coated	HC	2230805	869890
T28P_HCNNK_A120	Ang1	Solution	10nM Ang1 @ RT	HC	2091304	1265479
T28P_LCNNK_presort	—	—	—	LC	3085954	361233
T28P_LCNNK_V0.2	VEGF	Solution	0.2nM VEGF @ RT	LC	3192859	1930882
T28P_LCNNK_V0.2comp37	VEGF	Solution	0.2nM VEGF +1000x competition @ 37°C	LC	2068266	1241521
T28P_LCNNK_A20.2	Ang2	Solution	0.2nM Ang2 @ RT	LC	2799196	1350784
T28P_LCNNK_A20.2comp	Ang2	Solution	0.2nM Ang2 + 1000x competition @ RT	LC	1244163	123207
T28P_LCNNK_A1	Ang1	Plate	5ug/ml Ang1 coated	LC	1096430	59751
T28P_LCNNK_A120	Ang1	Solution	10nM Ang1 @ RT	LC	1828262	914787