Table 3.
Developability profiles of 5A12.1 Fab and 5 5A12.1 Fab variants. The melting temperature (Tm) of the 5A12.1 and five selected 5A12 Fab variants as determined by DSF is listed. In addition, non-specific binding properties of the IgGs were determined by baculovirus ELISA. The normalized baculovirus ELISA score is listed. The low binding and high binding antibody controls had normalized baculovirus ELISA score as 0.15 and 3.23, respectively.
| Clone | Tm (°C) | Baculovirus ELISA |
|---|---|---|
| T28P | 81.8 | 0.31 |
| HC.S30G | 80.8 | 0.4 |
| HC.Y58I | 82.2 | 0.78 |
| HC.Y58W | 82 | 0.51 |
| LC.S56Q | 82 | 0.35 |
| LC.H89M | 84.2 | 0.42 |