Figure 2.

Maintenance of VH and VL pairing during batch reformatting of a mini-library of 31 scFvs with known sequences to full-length IgG. Antibody amplification steps of the reformatting process were done using either standard aqueous PCR conditions (A) or using emulsion PCR where DNA templates are separated within water-in-oil droplets (B). scFv DNA sequences from each pool were identified by their VH and VL CDR3 sequences. Numbers in the table show IgG sequences observed with the certain VH and VL combinations indicated. IgG having the original VH-VL pairing are indicated by shading. Values in square brackets indicate the % correct pairing rate in each population from 86 or 87 scFv sequences analyzed.