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. 2017 Jun 14;9(6):996–1006. doi: 10.1080/19420862.2017.1337617

Table 1.

Comparison of variable domain sequences of phagemid scFv from Round 2 of selection and as VH and VL in IgG after batch reformatting to IgG.

 
PRE SiPF (phagemid)
SiPF Vector
  Library 1
Library 2
Library 1
Library 2
              λ       λ   κ  
  λ 352 κ λ 264 κ VH VL VH κ VH VL VH VL
Total reads 229   5 136   27 176 176 176 176 176 176 176 176
Full length, in-frame scFv   66%     62%   157 166 148 167 161 165 158 164
              89% 94% 84% 95% 91% 94% 90% 93%