Table 2.
V-domains pairing for generation of humanized antibody fragments and functional characteristics.
| Variant |
VL domain |
VH domain |
Binding to GPVI-Fc |
| ACT 001 | 9O12 VL | 9O12 VH | Functional |
| ACT 002 | humanized | first generation | Functional |
| ACT 004 | VL2b | VH1 | Not functional |
| ACT 005 | Binds PpL | VH2 | Functional |
| ACT 006 | VH3 | Functional | |
| ACT 007 | VH4 | Not functional | |
| ACT 008 | VL3b | VH1 | Not functional |
| ACT 009 | Does not bind PpL | VH2 | Not functional |
| ACT 010 | VH3 | Functional | |
| ACT 011 | VH4 | Not functional | |
| ACT 012 | VL7b | VH2 | Not functional |
| ACT 013 | VH3 | Not functional | |
| ACT 014 | VH4 | Not functional | |
| ACT 015 | VL8b | VH1 | Not functional |
| ACT 016 | Binds PpL | VH2 | Functional |
| ACT 017 | VH3 | Functional | |
| ACT 018 | VH4 | Not functional |