Figure 3.

CALY-002 inhibits IL-15–induced survival and STAT5 phosphorylation of type II RCD patient IEL cell lines. Type II RCD IEL cell lines were cultured with medium (white bars), IL-15 (black bars), IL-15 with CALY-002 (hatched bars) or IL-15 with control isotype (gray bars) for 48h. (A) Percentages of apoptotic cells in cultured IELs were determined by labeling using an AnnexinV-APC/PtdIns Kit and (B) phosphorylated STAT5 was stained with PEcy7-labeled anti-p-STAT5 antibody. Results were transformed as percentage of control (medium) for the percentage of apoptotic cells or the MFI of phosphorylated STAT5 intracellular expression induced by IL-15, and expressed as mean ± SD of results obtained with 3 different type II RCD IEL cell lines.