Table 1.

Characterization of the binding affinity, species specificity and selectivity of CALY-002. Kinetic interaction of CALY-002 with recombinant IL-15 was measured by surface plasmon resonance. Dissociation (k_off) and association (k_on) rate constants were obtained by nonlinear regression analysis. K_D = k_off /k_on. Selectivity and selectivity of CALY-002 toward recombinant human, monkey, mouse and rat IL-15 as well as human IL-2 was tested by ELISA. Whenever appropriate, the half-maximum binding concentration of CALY-002 (BC₅₀) was calculated. Results are expressed as the mean of 3 independent experiments ± SD.

	Surface plasmon resonance			ELISA binding assays
Assays	kon	Koff	KD	BC50	BC50	Binding	Binding	Binding
	(1/Ms)	(1/s)	(pM)	Human	Monkey	rat	mouse	human
				IL-15 (nM)	IL-15 (nM)	IL-15	IL-15	IL-2
CALY-002 characteristics	1.15 × 106	1.07 × 10−5	9.4	0.55 ± 0.46	0.43 ± 0.11	No Binding	No Binding	No Binding