Figure 6.
In vitro binding capabilities of the Fcs and Fabs from papain-digested mAb-PFM monomer and HMW species, the (Fab)2 from papain-digested mAb-PFM HMW species, the (Fab’)2 from pepsin-digested mAb-PFM monomer, to the antigen of mAb-PFM by SPR (Biacore). The sensorgrams of the 2 Fc samples (isolated from the digests of monomer and HMW) and the 2 Fab samples (isolated from the digests of monomer and HMW) are respectively superimposable. All fragments were purified using protein A column followed by SE-HPLC. Each displayed sensorgram is the average of triplicate measurements, corrected for non-specific binding with a blank flow cell.