Table 2.
Mass determination of the papain fragments.
| Papain Fragmenta | Theoretical Massb (Da) | Observed Massc (Da) | Weight-average Molar Massd (kDa) |
|---|---|---|---|
| Mon-Fab | 46315.5 | 46315.4 | 45 |
| HMW-Fab | 46315.5 | 46315.3 | 46 |
| HMW-(Fab)2 | 92630.8 | 46315.4 | 88 |
| Mon-Fc | 52819.3 | 52819.7 | 58 |
| HMW-Fc | 52819.3 | 52819.6 | 59 |
a
Papain-digested fragments of mAb-PFM monomer (Mon-Fab, Mon-Fc) and of mAb-PFM HMW species (HMW-Fab, HMW-(Fab2), HMW-Fc). All fragments were purified using protein A affinity chromatography and SE-HPLC, see Fig. 5.
b
Calculated for the major species observed in the SEC fraction due to heterogeneous proteolysis.
c
Mass of the major species in the SEC fraction by RP-HPLC/ESI-QTOF MS.
d
Determined by SEC-MALS.