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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Appl Microbiol. 2017 Aug;123(2):544–555. doi: 10.1111/jam.13493

FIGURE 2. Most stable secondary structure of exo probe targeting K. pneumoniae.

FIGURE 2

ΔG = −3.62 kcal mol−1; the transparent dotted circle (position 28) marks the nucleotide position replaced by THF and eventually cleaved by exonuclease III. RPA salt conditions − 14 mM Mg2+, 100 mM K+ – were used for secondary structure prediction.